Ebadi Atyeh; Moravvej Hamideh; Hejazi Somayeh; Younespour Shima; Ghalamkarpour Fariba
Volume 16, Issue 3 , 2013, , Pages 89-93
Abstract
Background: Despite great advances in therapeutic regimens,complete repigmentation in vitiligo is still out of reach. The aimof this assay was to study the efficacy and tolerability of repeatedneedling combined with narrowband ultraviolet B (NBUVB)therapy in the treatment of generalized, stable, refractory ...
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Background: Despite great advances in therapeutic regimens,complete repigmentation in vitiligo is still out of reach. The aimof this assay was to study the efficacy and tolerability of repeatedneedling combined with narrowband ultraviolet B (NBUVB)therapy in the treatment of generalized, stable, refractory vitiligo.Method: Twenty-six patients with stable generalized intractablevitiligo were recruited in before/after clinical trial. The patientsreceived needling for selected patches weekly for 12 weeks. Allthe patients received NBUVB phototherapy three times a week.Repigmentation improvement was assessed by two blindedinvestigators at weeks 4, 8 and 12. Tolerability was assessed bya 5-point scale.Result: Twenty-two patients (16 women and 6 men) with a mean(SD) age of 34.41 (12.75) years completed the 12-week therapyperiod. In comparison with the baseline, improvement in themean percentage of repigmentation was 7.10±10.15, 12.95±20.29,and 15.57±17.38 at week 4, 8 and 12 of therapy, respectively.A significant improvement was observed in repigmentationpercentages in the three time points (p
Barikbin Behrooz; Qeisari Mehdi; Saeedi Marjan; Esmailiazad Mitra; Moravvej Hamideh; Yousefi Maryam; Ahmadi Amrollah
Volume 13, Issue 1 , 2010, , Pages 16-19
Abstract
Background: Psoriasis is a common inflammatory skin disorder. There has been considerable interest in herbal medicine as a treatment for psoriasis. In a previous study HESA-A, a marine-herbal drug, was found to be beneficial in the treatment of psoriasis vulgaris. The aim of this study was to assess ...
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Background: Psoriasis is a common inflammatory skin disorder. There has been considerable interest in herbal medicine as a treatment for psoriasis. In a previous study HESA-A, a marine-herbal drug, was found to be beneficial in the treatment of psoriasis vulgaris. The aim of this study was to assess the efficacy and tolerability of HESA-A in patients with psoriasis. Methods: Nineteen patients with a mean PASI score of 13.04±6.14 (min=3.90, max=27.70) were recruited to receive daily dosage of 30 mg/kg of HESA-A tablets for at least 4 weeks. The patients were followed every two weeks for determining PASI score changes and drug side effects until the end of the study. Result: At the end of the study, the mean PASI score of the patients reduced to 9.60±5.30 (min=0.90, max=19.1). PASI score reduced in 14 patients (73.7%) and increased in 5 patients (26.3%) during the study. Two patients achieved 75 to 100% improvement in PASI score and two achieved 50 to 75%. In 10 patients (52.6%), 1 to 50% improvement in the PASI score was detected. There was a statically significant correlation between the duration of treatment and PASI improvement. (P-value = 0.024) Conclusion: Although this drug was very safe and tolerable, our study did not find rapid and acceptable efficacy in the treatment of chronic plaque psoriasis as shown in a previous study. According to our findings, HESA-A was more effective when used for a prolonged time and in respect of the safety profile of HESA-A, we can use this drug as a maintenance or adjuvant therapy for chronic plaque psoriasis in longer terms.
Moravvej Hamideh; Rad Mahnaz Mahmoudi; Toossi Parviz; Khorasani Mohammad Taghi; Mirzadeh Hamid
Volume 12, Issue 4 , 2009, , Pages 111-116
Abstract
Background: Fibroblasts are mesenchymal cells that can be readily cultured in the laboratory and play a significant role in epithelialmesenchymal interactions, secreting various growth factors and cytokines that have a direct effect on epidermal proliferation, differentiation and formation of extracellular ...
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Background: Fibroblasts are mesenchymal cells that can be readily cultured in the laboratory and play a significant role in epithelialmesenchymal interactions, secreting various growth factors and cytokines that have a direct effect on epidermal proliferation, differentiation and formation of extracellular matrix. They have been incorporated into various tissue-engineered and used for a variety of clinical applications, including the treatment of burns, chronic venous ulcers and several other clinical applications in dermatology and plastic surgery. Method: Isolated fibroblasts by the enzymatic process from foreskin were cultivated successively in a culture medium to establish cell banking. Foreskin and the last subcultured cells were checked for HBV, HCV, HIV, HSV I, HSV II, HTLV I, HTLV II, EBV, CMV, Treponema Pallidum, Mycoplasma sp. and Clamydia. The 1st, 5th and 10th subcultured cells were processed for immunocytochemistry studies using a panel of monoclonal antibodies including antibodies to MHC class I & II antigens for ensuring the elimination of superficial cell antigens during cultivation. Subcultured cells were karyotyped to find any chromosomal abnormalities. The best passages were chosen for culturing on silicone sheets provided by the Iran Polymer and Petrochemical Institute. Results: Evaluation for bacteria and viruses by molecular methods was negative. Karyotyping of cultured fibroblasts after the 10th passage showed some abnormalities. HLA expression was imperceptible in the cells obtained from the 10th sub-culture. The best passages were from 5th to 10th for banking and culturing on silicone sheets. Conclusion: Expression of HLA on fibroblast surfaces was diminished during subculturing. To prevent chromosomal abnormalities in fibroblast passaging, we should select the best colony that is expected to be chromosomally stable with the least antigenicity. In our study, the 5th to 10th sub-cultures were the best cells for the purpose of grafting and acceleration of the wound healing.
Yousefi Maryam; Barikbin Behrooz; Givrad Soudabeh; Moravvej Hamideh; Khoshnoudi Roxanna
Volume 12, Issue 4 , 2009, , Pages 139-140
Moravvej Hamideh; Rad Mahnaz Mahmoodi; Zali Hakimeh; Nabai Leila; Toossi Parviz
Volume 12, Issue 1 , 2009, , Pages 4-8
Abstract
Background: Human fibroblasts are the part of the dermis that secrete extracellular matrix for the purpose of tissue repair. Culturing fibroblasts, which leads to formation of a monolayer of these cells, is used for treating various conditions including thermal burns and other skin defects such as diabetic ...
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Background: Human fibroblasts are the part of the dermis that secrete extracellular matrix for the purpose of tissue repair. Culturing fibroblasts, which leads to formation of a monolayer of these cells, is used for treating various conditions including thermal burns and other skin defects such as diabetic and varicose vein leg ulcers. Therefore, we aimed at developing a fibroblast bank to accomplish multiple goals including skin repair in defects such as burns and ulcers and also performing various research projects on these cells in order to further study of the mechanisms involved in wound healing, rejuvenation and medication effects. Method: We initially developed primary cultures of skin fibroblasts in a DMEM medium. These primary cultures were formed by washing and trypsinizing foreskin specimens followed by separation of epidermis from dermis and cutting the dermis into small pieces. In about 10 days, a monolayer of fibroblasts was formed. Result: We were able to develop the fibroblast bank successfully and to initiate other projects utilizing this bank. Conclusions: With these cultured cells, we would be able to perform different research projects including studying the mechanisms of wound healing, rejuvenation, drug affects, inflammatory mediators, growth factors, etc. Moreover, further progress in this field will result in our independence from requesting these cells from external sources.
