Mohamad Afshar; Mahmoudreza Jafari; Mohamadmehdi Hasanzadeh Taheri; Mohsen Khorashadizadeh; Hamide Taheri Olyayie
Abstract
Background: Curcumin (diferuloylmethane) is one of the mostactive components of turmeric. This herbal compound has antiinflammatoryand positive wound-healing impacts. The principalobjective of this study was to evaluate the impacts of curcuminnanoliposomes on cell viability and motility of mouse fibroblastNIH ...
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Background: Curcumin (diferuloylmethane) is one of the mostactive components of turmeric. This herbal compound has antiinflammatoryand positive wound-healing impacts. The principalobjective of this study was to evaluate the impacts of curcuminnanoliposomes on cell viability and motility of mouse fibroblastNIH 3T3 cells and its wound healing effects on second-degreeskin burns in BALB/c mice.Methods: Mature male BALB/c mice (n = 48) were dividedinto 4 groups (n = 12 per group). Group one received curcuminnanoliposome ointment; the positive and negative control groups(groups 2&3) were treated with silver sulfadiazine and placebo,respectively, and group four (sham) received no treatment. Theburn wound was created by a metal device with a diameter of 1cm. Animals received treatment twice daily. On days 4, 7, 10, and14, deep anesthesia and a biopsy of the wound were performed,and a microscopic study and MTT assay were carried out.Results: Cellular studies on mouse fibroblast NIH-3T3 cellsshowed that low-dose curcumin nanoliposomes increased cellproliferation and motility at 8, 12, and 24 hours in comparisonwith the control group. In tissue samples of mice treated withcurcumin nanoliposome (day 14), less inflammation was observed,while granulation tissue formation, fibroblast proliferation,epithelialization, and collagen fiber synthesis increased significantlycompared with the control groups.Conclusion: Our study indicates the positive effects of curcuminnanoliposomes on the motility process of mouse fibroblast NIH-3T3 cells (in vitro) and on the inflammatory and proliferativephases (in vivo) of burn wound healing in mice.
Mohammad Afshar; Mohammadmehdi Hassanzadeh- Taheri; Mahmoud Zardast; Maryam Honarmand
Abstract
Background: Wound healing is one of the main problems faced by medical scientists. Nowadays, herbal compounds are used to accelerate the repairing process. Coumarin is a plant compound with anti-inflammatory and anti-oxidant effects. In the present study, the benefits of using coumarin ...
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Background: Wound healing is one of the main problems faced by medical scientists. Nowadays, herbal compounds are used to accelerate the repairing process. Coumarin is a plant compound with anti-inflammatory and anti-oxidant effects. In the present study, the benefits of using coumarin in accelerating wound healing were investigated in mice.Methods: Sixty male BALB/c mice were used. After making a linear wound on the dorsum of the animals, they were randomly divided into five equal groups: the first and second groups received topical cream of coumarin at concentrations of 1% and 2%; the third and fourth groups received nitrofurazone cream (positive control) and eucerin cream (negative control), respectively. The fifth group as the sham group was not treated. Then on days 4, 7, 10, and 14 of experiment, biopsies were performed on three mice from each group. Histological examination was performedusing hematoxylin and eosin and Masson trichrome staining. Data were analyzed by ANOVA and Tukey tests.Results: Inflammation significantly decreased in both experimental groups at days 4, 7, and 10, compared to the control groups. In the proliferation phase, fibroblast cells, granulation tissue formation, and epithelialization were significantly higher in both experimental groups than the control groups. In addition, collagen synthesis significantly increased in the experimental groups compared to the control groups.Conclusion: Topical application of coumarin had beneficial effects on different phases of wound healing in the skin of BALB/c mice.
Khalaji Naser; Khaffafi Behrang; Mokhtari Seyed Arman Seyed; Karimipour Mojtaba; Alizadeh Hamed
Abstract
Background: Nowadays, the rapid recovery of skin lesions and functional return are among the goals of researchers. The skin is the first defensive barrier against microorganisms in the body and its failure causes infection to spread in all systems of the body. By taking into account the contradictory ...
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Background: Nowadays, the rapid recovery of skin lesions and functional return are among the goals of researchers. The skin is the first defensive barrier against microorganisms in the body and its failure causes infection to spread in all systems of the body. By taking into account the contradictory results of previous studies on the impact of phototherapy on wound healing and also the considerable anti-oxidative properties of curcumin, this novel study was carried out with the aim of determining the histopathological impact of compact fluorescent light (CFL) and curcumin on the process of wound healing. Methods: Forty-eight adult male wistar rats were randomly divided into four groups. The control group received 2.0 ml of ethyl oleate, and the curcumin group received only 0.2 ml curcumin daily for 15 days via intraperitoneal injection. The fluorescent group received 0.2 ml of ethyl oleate daily for 15 days via intraperitoneal injection, and were exposed to CFL for 12 hours per day for 15 days. The curcumin plus fluorescent group received 0.2 ml curcumin daily for 15 days via intraperitoneal injection, and were exposed to CFL for 12 hours per day for 15 days. The size of the wound was measured by a scale ruler, and the morphology of the wound site was assessed. Results: The results of this study showed that the best percentage of repair was observed in the fluorescent group on days 6 and 15 (50±5 and 90±2, respectively), while the least repair was seen in the group receiving fluorescent plus curcumin (33±7). In the curcumin group, the wound healing was, not significantly (P=0.872) reduced on the sixth day, compared to the control group, whereas compared to the fluorescent and fluorescent plus curcumin groups, the reduction was significant (P?0.0001 and P=0.05, respectively). On the fifteenth day, however, the wound healing was significantly decreased in the curcumin group compared to the control and fluorescent groups (P?0.0001 and P?0.0001 respectively), while it was significantly increased compared to the fluorescent plus curcumin group (P?0.0001). In the fluorescent plud curcumin group, the wound healing was significantly reduced compared to the other groups on the fifteenth day (P?0.0001). Conclusion: Fluorescent alone resulted in wound healing, in contrast to the control and curcumin plus fluorescent groups. Accelerating the repair in this group is likely due to the increase in blood flow and helping the homeostasis to return to its primary state. The absence of wound healing in the curcumin group is probably due to the high dose of curcumin. Moreover, in the fluorescent plus curcumin group, the causes of no wound healing and weight loss were probably disorders in the inflammation process and spread of infection.
ZB Mousavi; M Meshki; A Hemmati; MS Veisi; R Rafiei
Volume 9, Issue 3 , 2006, , Pages 260-263
Abstract
Background and aim: Improvement of wound healing in normal subjects and in chronic ulcers has been always desired. The availablity and acceptability of herbal medicine led us to study the effect of topical 10% mucilage of quince seed in human skin.Materials and Methods: This double-blind clinical trial ...
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Background and aim: Improvement of wound healing in normal subjects and in chronic ulcers has been always desired. The availablity and acceptability of herbal medicine led us to study the effect of topical 10% mucilage of quince seed in human skin.Materials and Methods: This double-blind clinical trial has been conducted on 34 patients referring to Imam Hospital, clinic of dermatology for elective surgery or excisional biopsy of benign lesions. The patients were divided into 3 groups and their lesions were treated with either 10% quince mucilage or eucerin or left untreated. The wound surface was measured on the days 0, 3, 5, 7, 14 and 20. The data were analyzed by ANOVA.Results: The mean duration of complete healing was 10.72 days in quince mucilage, 15.62 days in eucerin, and 16.7 days in untreated group (P<0.05).Conclusion:10% quince mucilage ointment improved wound healing. Further and larger studies are needed to evaluate the quince mucilage effect and its possible application for patients suffering form chronic ulcers.
M Bayat; M.Gh Gol Mohammadi; FS Rezaei
Volume 8, Issue 6 , 2006, , Pages 475-481
Abstract
Background and aim: Low-power lasers relief pain in some musculoskeletal disorders and accelerate wound healing process. However, there are few reports on effects of low-power lasers on mast cells. In this study the effects of low-power gallium aluminium arsenide laser (Ga.Al.As.laser) radiation on number ...
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Background and aim: Low-power lasers relief pain in some musculoskeletal disorders and accelerate wound healing process. However, there are few reports on effects of low-power lasers on mast cells. In this study the effects of low-power gallium aluminium arsenide laser (Ga.Al.As.laser) radiation on number and degranulation of mast cells of open skin wound bed of rats were studied using quantitative histological methods.Materials and Methods: Forty-six male rats were randomly divided into experimental and control groups. Each group divided into 3 subgroups based on duration of study: 4 , 7 and 15 days. Under general anesthesia and sterile conditions one circular full thickness skin wound was made on the doesum of neck of each rat. The wounding day was considered as the day zero. From the day one, experimental rats received Ga. Al. Ar. laser radiation at a energy density of 1.2 J/cm2. After doing daily treatments, on days 4, 7 and 15, rats were killed by ether and samples were obtained from wound bed and normal adjacent skin from each rat. Samples were fixed in formalin saline and were prepared for routine histological study. Sections were stained by 0.1% watery solution of toluidine blue and total number of mast cells and their grades (one, two and three) were counted. In grade one, mast cell is intact, in grade two some granules have been extruded from the cell and in the mast cells of grade 3, degranultion is more extensive and widespread. Data were analysed by Student t test.Results: On the day 4, total number and grade one mast cells were significantly higher in the control group (P<0.01). On days 7 and 15, total number of mast cells and their grades were higher in the experimental group. The grade 2 mast cells on day 7 and grade 1 mast cells on day 15 of experimental group was significantly higher than control group (P<0.05).Conclusion: Low-power gallium aluminium arsenide laser irradiation on open skin wound of rats reduced significantly total number of mast cells and intact ones at inflammatory phase, and 90 significantly increased active mast cells at proliferation, and intact ones at remodeling phases of the wound healing process.
M Bayat; Z Asgari Moghaddam; M Rakhshan; FS Rezaei
Volume 8, Issue 5 , 2005, , Pages 373-378
M Bayat; SN Razavi; A Hosseini
Volume 4, Issue 2 , 2001, , Pages 3-8
Abstract
Background: Positive effects of Kombucha tea (An old folkloric drink of Asian people) on wound healing process have been observed. Objective: To study its effects on the number of mast cells of wound bed. Materials and Methods: The study design was experimental. 42 male adult rats were randomly divided ...
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Background: Positive effects of Kombucha tea (An old folkloric drink of Asian people) on wound healing process have been observed. Objective: To study its effects on the number of mast cells of wound bed. Materials and Methods: The study design was experimental. 42 male adult rats were randomly divided into control and experimental groups. Each group was divided into 3 subgroups. The time of study of first subgroups were 4 days (Represents inflammation phase of wound healing process), of second subgroups were 7 days (Proliferation phase) and of third subgroups were 15 days (Remodeling phase) after wounding. Both groups drank Kombucha tea for 30 days. Then under general anesthesia and sterile conditions, a full thickness wound was made on the dorsum of all rats. The wounding day was considered as day zero. Rats of experimental group drank Kombucha tea after surgery until they were killed by ether at the end of above-mentioned durations. Samples were obtained from rats and prepared for light microscopical study and stained with 1% watery solution of toluidine blue. Mast cells and its grades (According to it’s degranulations) were counted. Data were analyzed by students t test method. Results: Main results were: 1) Total number of most cells of experimental group decreased from 7th day toward 15th day and on 15th day, the difference with control group was significant (P<0.01); 2) In most cases the numbers of mast cells of experimental group were lower than control group and the difference were significant for the 4th day grade 1, 15th day grade 2 (P<0.05) and total number of grades 2 and 3 (P<0.01). Conclusion: Daily consumption of Kombucha tea by rats caused a decreased number of mast cells during skin wound healing and the difference with control group at remodeling phase was significant.
M Bayat
Volume 3, Issue 2 , 2000, , Pages 23-26
Abstract
Introduction: Positive effects of topical application of honey on open skin wounds have been observed, Mast cell are playing role in normal wound healing and its disturbances but the role of mast cells in wound healing in existence of honey is uncertain. Objective: The aim of this investigation was to ...
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Introduction: Positive effects of topical application of honey on open skin wounds have been observed, Mast cell are playing role in normal wound healing and its disturbances but the role of mast cells in wound healing in existence of honey is uncertain. Objective: The aim of this investigation was to study the effects of two times per day topical application of honey on the number of mast cells in skin wounds. Materials and Methods: 33 male rats were randomly divided into experimental and control groups. Under general anesthesia and sterile conditions, one round full thickness skin wound was made on the dorsum of neck of each rat. The diameter of wounds was about 3cm. Unboiled honey was applied topically on wounds twice per day from surgery day until certain days. On the fourth, seventh, and fourteenth days after surgery according to inflammation, proliferation and remodeling phases of wound healing process, samples were obtained from wound tissue and adjacent skin after rats were killed with ether. Samples were prepared for routine histological study and stained with 1% aqueous toluidine blue. Mast cells of wounds were counted and analyzed by Mann Whitney U test. Results: The mean of mast cells was lower in experimental group in three above-mentioned days. The differences were statistically significant on days 4 and 7 (P<0.05 for fourth day, P<0.01 for seventh day, and P>0.05 for fourteenth day). Conclusion: Twice per day application of honey on open full thickness skin wounds could reduce the number of mast cells significantly, in inflammatory and proliferative phases of wound healing.