Mohamad Afshar; Mahmoudreza Jafari; Mohamadmehdi Hasanzadeh Taheri; Mohsen Khorashadizadeh; Hamide Taheri Olyayie
Abstract
Background: Curcumin (diferuloylmethane) is one of the mostactive components of turmeric. This herbal compound has antiinflammatoryand positive wound-healing impacts. The principalobjective of this study was to evaluate the impacts of curcuminnanoliposomes on cell viability and motility of mouse fibroblastNIH ...
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Background: Curcumin (diferuloylmethane) is one of the mostactive components of turmeric. This herbal compound has antiinflammatoryand positive wound-healing impacts. The principalobjective of this study was to evaluate the impacts of curcuminnanoliposomes on cell viability and motility of mouse fibroblastNIH 3T3 cells and its wound healing effects on second-degreeskin burns in BALB/c mice.Methods: Mature male BALB/c mice (n = 48) were dividedinto 4 groups (n = 12 per group). Group one received curcuminnanoliposome ointment; the positive and negative control groups(groups 2&3) were treated with silver sulfadiazine and placebo,respectively, and group four (sham) received no treatment. Theburn wound was created by a metal device with a diameter of 1cm. Animals received treatment twice daily. On days 4, 7, 10, and14, deep anesthesia and a biopsy of the wound were performed,and a microscopic study and MTT assay were carried out.Results: Cellular studies on mouse fibroblast NIH-3T3 cellsshowed that low-dose curcumin nanoliposomes increased cellproliferation and motility at 8, 12, and 24 hours in comparisonwith the control group. In tissue samples of mice treated withcurcumin nanoliposome (day 14), less inflammation was observed,while granulation tissue formation, fibroblast proliferation,epithelialization, and collagen fiber synthesis increased significantlycompared with the control groups.Conclusion: Our study indicates the positive effects of curcuminnanoliposomes on the motility process of mouse fibroblast NIH-3T3 cells (in vitro) and on the inflammatory and proliferativephases (in vivo) of burn wound healing in mice.
Razieh Bokaiean; Mahnoush Momeni; Parisa Sabrjoo; Mostafa Dahmardehei; Maryam Roham; Hossein Rahber
Abstract
Background: Active Leptospermum honey has non-peroxide antibacterial and anti-inflammatory effects, rendering it suitable for wound healing. Leptospermum honey is endemic in New Zealand belonging to the manuka bush (Leptospermum scoparium). The objective of the present research was to compare the efficacy ...
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Background: Active Leptospermum honey has non-peroxide antibacterial and anti-inflammatory effects, rendering it suitable for wound healing. Leptospermum honey is endemic in New Zealand belonging to the manuka bush (Leptospermum scoparium). The objective of the present research was to compare the efficacy of manuka honey dressing with conventional dressing regarding skin graft donor sites following a burn injury.Methods: This study was carried out in the department of surgery, Iran University of Medical Sciences, Tehran, Iran. It is a noncontrolled prospective trial, and an open-label study, analyzing Leptospermum honey and conventional dressing for the treatment of donor site areas for skin grafts. Data were collected from 15 eligible patients with burn wound. Two independent donor sites were formed, one of which was treated with active Leptospermum honey dressing and the other covered through the conventional method. Further collected was information regarding subjects’ demographics, self-reported pain (VAS scale), wound surface areas and bacterial wound culture.Results: In the treatment of skin graft donor sites, honey proved to be less painful compared with the conventional group (P=0.001). Three and seven days following treatment, a significant decrease was observed in the mean wound surface areas in the honey group (P=0.001). There was no significant difference between honey and conventional dressings with regards to the rate of infection (20% in honey dressing versus 40% in conventional group; P=0.068).Conclusions: Active Leptospermum honey dressing accelerates the healing process, decreases pain and has antimicrobial activity and can be used for care of skin graft donor sites.
Moravvej Hamideh; Rad Mahnaz Mahmoodi; Zali Hakimeh; Nabai Leila; Toossi Parviz
Volume 12, Issue 1 , 2009, , Pages 4-8
Abstract
Background: Human fibroblasts are the part of the dermis that secrete extracellular matrix for the purpose of tissue repair. Culturing fibroblasts, which leads to formation of a monolayer of these cells, is used for treating various conditions including thermal burns and other skin defects such as diabetic ...
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Background: Human fibroblasts are the part of the dermis that secrete extracellular matrix for the purpose of tissue repair. Culturing fibroblasts, which leads to formation of a monolayer of these cells, is used for treating various conditions including thermal burns and other skin defects such as diabetic and varicose vein leg ulcers. Therefore, we aimed at developing a fibroblast bank to accomplish multiple goals including skin repair in defects such as burns and ulcers and also performing various research projects on these cells in order to further study of the mechanisms involved in wound healing, rejuvenation and medication effects. Method: We initially developed primary cultures of skin fibroblasts in a DMEM medium. These primary cultures were formed by washing and trypsinizing foreskin specimens followed by separation of epidermis from dermis and cutting the dermis into small pieces. In about 10 days, a monolayer of fibroblasts was formed. Result: We were able to develop the fibroblast bank successfully and to initiate other projects utilizing this bank. Conclusions: With these cultured cells, we would be able to perform different research projects including studying the mechanisms of wound healing, rejuvenation, drug affects, inflammatory mediators, growth factors, etc. Moreover, further progress in this field will result in our independence from requesting these cells from external sources.
J Movaffagh; MR Jafari; MH Amouzegar; SA Tabatabaei
Volume 9, Issue 1 , 2006, , Pages 2-16