Mohammad Rahmati-Roodsari; Maryam Ranjbar; Azadeh Rakhshan
Volume 18, Issue 2 , 2015, , Pages 61-64
Abstract
Background: Some studies have reported a correlation between adnexal tumors and human papilloma virus (HPV) infection. We performed this study to determine the incidence of HPV infection in adnexal tumor samples to further clarify any potential link between HPV and the pathogenesis of adnexal ...
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Background: Some studies have reported a correlation between adnexal tumors and human papilloma virus (HPV) infection. We performed this study to determine the incidence of HPV infection in adnexal tumor samples to further clarify any potential link between HPV and the pathogenesis of adnexal tumors.Methods: A total of 65 formalin-fixed, paraffin-embedded, tissue samples with a histopathologically confirmed diagnosis of adnexal tumors, obtained between 2006 and 2011, were retrieved and evaluated by polymerase chain reaction (PCR) for the presence of different types of HPV.Results: The samples included 29 sebaceous gland tumors, 18 sweet gland tumors, and 18 follicular epithelium tumors. The HPV DNA was only detected in four out of 18 sweet gland tumors (22.2%), i.e. in 6.2% of all evaluated tumors. HPV-6 and HPV-54 were detected in one and three samples, respectively.Conclusion: Considering the low rate of HPV in adnexal tumors, the probability of an association between HPV and adnexal tumor seems slim.
Pezeshkpoor Fakhrozaman; Tohidi Mohammad; Layegh Pouran; Shahabi Majid
Volume 16, Issue 2 , 2013, , Pages 45-48
Abstract
Background: Accurate diagnosis of cutaneous leishmaniasis wouldavoid unnecessary treatment and scar formation. Direct smear isthe most common method for the diagnosis of this disease but itsnegative result could not rule out the infection; so, the need formore sensitive methods is obvious. We conducted ...
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Background: Accurate diagnosis of cutaneous leishmaniasis wouldavoid unnecessary treatment and scar formation. Direct smear isthe most common method for the diagnosis of this disease but itsnegative result could not rule out the infection; so, the need formore sensitive methods is obvious. We conducted this study tocompare the efficiency of direct skin smear with smears preparedby fine needle aspiration (FNA) using polymerase chain reaction(PCR) as a reference method.Methods: In this cross-sectional study, which was conductedduring two years from May 2008 to May 2010, 33 patients withsuspicious acute cutaneous leishmaniasis based on clinical studieswere randomly selected. Direct skin smears and FNA smearswere taken from each patient and PCR was performed on biops
Najmossadat Atefi; Mehdi Tabaie; Samile Noorbakhsh; Azarmidokht Tabatabaie; Mohammadreza Rezaee; Masomeh Rohaninasab
Volume 15, Issue 1 , 2012, , Pages 1-3
Abstract
Background: T cell activation is discussed as an important factor in the pathogenesis of psoriasis. Recently, a lot of attention has been paid to the role of superantigens in T cell activation in the pathogenesis of psoriasis. In this study, the role of staphylococcal superantigens in the pathogenesis ...
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Background: T cell activation is discussed as an important factor in the pathogenesis of psoriasis. Recently, a lot of attention has been paid to the role of superantigens in T cell activation in the pathogenesis of psoriasis. In this study, the role of staphylococcal superantigens in the pathogenesis of psoriasis with identification of staphylococcal toxin levels in the skin lesions of patients with chronic plaque psoriasis has been studied. Method: In this case-control study, biopsies were taken from the skin of 40 patients with chronic plaque type psoriasis and 40 controls. Staphylococcal superantigens such as staphylococcus enterotoxin A, staphylococcus enterotoxin C, and toxic shock syndrome toxin 1 were investigated using polymerase chain reaction. Result: Staphylococcus aureus was isolated from 6.5% of the psoriasis patients and 2.5% of the individuals in the control group and all of them were toxin producer. There was a significant difference between controls and patients. Conclusion: Bacterial superantigens probably play an important role in the pathogenesis of chronic plaque type psoriasis.
Soheila Nassiri; Mojdeh Sarlak; Farahnaz Bidari Zereh Posh; Siamak Mirab Samiee; Farzaneh(Batool) Rahimi; Mehdi Qaisari
Volume 12, Issue 2 , 2009, , Pages 52-55
Abstract
Background: Anogenital wart is a common sexually transmitted disease affecting both genders. The aim of this study was to evaluate human papilloma virus types of anogenital warts with Polymerase Chain Reaction (PCR). Method: 65 formalin fixed paraffin embedded (FFPE) tissue blocks of the anogenital warts ...
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Background: Anogenital wart is a common sexually transmitted disease affecting both genders. The aim of this study was to evaluate human papilloma virus types of anogenital warts with Polymerase Chain Reaction (PCR). Method: 65 formalin fixed paraffin embedded (FFPE) tissue blocks of the anogenital warts obtained from the pathology lab of Loghman Hakim hospital were included in this study. Results: In 27 samples (41.5%), human papilloma virus was detected by PCR. In addition, low risk HPV types (6, 11) were identified in all samples without any positivity for high risk HPV types (16, 18, 31 and 33). Conclusion: By finding low risk HPV types in all samples, it could be concluded that Anogenital warts seems not to be precursors of anogenital malignancies.
Hejazi Seyed Hossein; Mokhtarian Kobra; Eslami Gilda; Salehi Rasoul; Mohammad Ali Nilforoushzade; Leila Shirani; Sedigheh Saberi
Volume 10, Issue 3 , 2007, , Pages 229-235
Abstract
Background and aim: Cutaneous leishmaniasis (CL) is a health problem of many countries in tropical and subtropical regions including Iran. Isfahan province is one of the foci of CL with the highest prevalence in Iran. This study was done to identify the species of Leishmania isolated from the patients ...
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Background and aim: Cutaneous leishmaniasis (CL) is a health problem of many countries in tropical and subtropical regions including Iran. Isfahan province is one of the foci of CL with the highest prevalence in Iran. This study was done to identify the species of Leishmania isolated from the patients in Ghohab Mohammad Abad, located in southwest of Isfahan which is a new foci of CL. Identification of the causative agent of CL is required to plan control measures and therapeutic strategies.Materials and methods: All residents of the village (18,477 individuals) were visited and interviewed to recruit patients with active lesion of CL. Direct samples and culture were taken from the suspected lesions for isolation and identification of Leishmania species. Identification was performed using kDNA minicircles in a PCR manner. Results: Forty three patients with suspected CL lesion were recruited and 25 parasitologically proven cases were identified. Eighteen isolates were used for identification and 7 isolates were excluded due to fungi contamination. All 18 isolates were characterized by PCR amplification to be Leishmania major.Conclusion: The etiologic agent of the CL in the region was identified to be L.major. Larger studies are needed to confirm that L. major is the rule etiologic agent of CL in this region.
