Mirhendi Seyed Hossein; Hedayati Mohammad Taghi; Omidi Khoshghadam; Zand Niloofar Jalali; Didehdar Mojtaba; Afshar Parvaneh
Volume 10, Issue 3 , 2007, , Pages 73-228
Abstract
Background and aim: Dermatophytosis (tinea, ringworm) is the infection of skin, hair or nail that is caused by various keratinophilic fungi (dermatophytes). Dermatophytosis is a common infection throughout the world including all parts of Iran. As conventional laboratory procedures for identification ...
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Background and aim: Dermatophytosis (tinea, ringworm) is the infection of skin, hair or nail that is caused by various keratinophilic fungi (dermatophytes). Dermatophytosis is a common infection throughout the world including all parts of Iran. As conventional laboratory procedures for identification of different dermatophytes are slow or lack specificity, more rapid and reliable methods are still required.Materials and methods: Dermatophyte fungi were isolated from patients with dermatophytosis and preliminarily identified by macroscopic and microscopic morphological criteria. Total cellular DNA was extracted from isolates using conical grinder. ITS1-5.8s-ITS2 region of rDNA region was amplified by the universal fungal primers ITS1 and ITS4 and digested with Eco RII enzyme.Results: 650-750 bp band was produced , as expected. Digestion of the PCR products with the restriction enzyme EcoRII produced different electrophoretic pattern and allowed us the identification and differentiation of common pathogenic dermatophytes including Trichophyton rubrum, T. interdigital, T. mentagrophytes, T. tonsurans, T.violaceum, T. schoenleinii, T. verrucosum, M.canis, M.gypseum and Epidermophyton floccosum. Conclusion: It seems that this PCR-restriction enzyme (PCR-RE) profile is a rapid and reliable tool for discrimination of important dermatophytes and can be an applicable method in reference medical mycology laboratories for diagnostic, as well as for large-scale epidemiological purposes.
Hejazi Seyed Hossein; Mokhtarian Kobra; Eslami Gilda; Salehi Rasoul; Mohammad Ali Nilforoushzade; Leila Shirani; Sedigheh Saberi
Volume 10, Issue 3 , 2007, , Pages 229-235
Abstract
Background and aim: Cutaneous leishmaniasis (CL) is a health problem of many countries in tropical and subtropical regions including Iran. Isfahan province is one of the foci of CL with the highest prevalence in Iran. This study was done to identify the species of Leishmania isolated from the patients ...
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Background and aim: Cutaneous leishmaniasis (CL) is a health problem of many countries in tropical and subtropical regions including Iran. Isfahan province is one of the foci of CL with the highest prevalence in Iran. This study was done to identify the species of Leishmania isolated from the patients in Ghohab Mohammad Abad, located in southwest of Isfahan which is a new foci of CL. Identification of the causative agent of CL is required to plan control measures and therapeutic strategies.Materials and methods: All residents of the village (18,477 individuals) were visited and interviewed to recruit patients with active lesion of CL. Direct samples and culture were taken from the suspected lesions for isolation and identification of Leishmania species. Identification was performed using kDNA minicircles in a PCR manner. Results: Forty three patients with suspected CL lesion were recruited and 25 parasitologically proven cases were identified. Eighteen isolates were used for identification and 7 isolates were excluded due to fungi contamination. All 18 isolates were characterized by PCR amplification to be Leishmania major.Conclusion: The etiologic agent of the CL in the region was identified to be L.major. Larger studies are needed to confirm that L. major is the rule etiologic agent of CL in this region.