Tafti Hossein Sadeghi; Ahmadikia Kazem; Rashidian Sara; Hekmatimoghaddam Seyed Hossein; Yazdi Zeinab Ayubi
Volume 20, Issue 2 , 2017, , Pages 37-42
Abstract
Background: Onychomycosis, the fungal infection of the toenails or fingernails, is caused by three major groups of fungi including dermatophytes, yeasts and non-dermatophyte molds. The objective of the present study was to determine the incidence of onychomycosis and to identify the causative fungi during ...
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Background: Onychomycosis, the fungal infection of the toenails or fingernails, is caused by three major groups of fungi including dermatophytes, yeasts and non-dermatophyte molds. The objective of the present study was to determine the incidence of onychomycosis and to identify the causative fungi during a one year period in Yazd, Iran. Materials and Methods: From Apr 2013 to Apr 2014 a total of 273 patients with suspected dermatophytosis were included in this study. Nail-clipping specimens of 71 clinically diagnosed cases of onychomycosis were obtained for mycological examination (KOH preparation and fungal culture). Identification of mycelial isolates was based on morphological appearance and microscopic characteristic of the colony. Supplementary methods for identification of dermatophytes were employed. The species of yeasts were identified by germ-tube and chlamydospore test, as well as colony color on chromogenic CHROMagar Candida medium, and the assimilation profile in API 20C Aux system. Results: Of the 71 patients affected by nail disorders, 26 (36.6%) patients of onychomycosis including 54.9% male and 45.1% female (20 fingernails, 6 toenails) via direct examination and/or culture methods were diagnosed. saprophytic fungi were the most prevailing causative agents of onychomycosis and account for up to 69.2%(n=18) of cases, yeasts and dermatophytes were identified as causative agents of onychomycosis in 7 (26.9%) patients and 1 patient (3.8%), respectively. Distribution of fungal isolates was as follows: Aspergillus niger (26.9%), A. fumigatus (19.2%), Candida albicans (15.3%), A. flavus (11.5%), C. tropicalis (7.6%), Penicillium sp. (7.6%), C. dubliniensis (3.8%), Trichophyton mentagrophytes (3.8%) and Fusarium sp. (3.8%). Conclusion: Because of considerable prevalence of onychomycosis, necessity for a careful mycological examination in patients with nail disorders is highlighted.
Sarvtin Mehdi Taheri; Hedayati Mohammad Taghi; Abastabar Mahdi; Shokohi Tahereh
Volume 17, Issue 4 , 2014, , Pages 134-137
Abstract
Background: Psoriasis is an immune mediated skin disorderwhich is mainly characterized by abnormal proliferation anddifferentiation of keratinocytes. It is believed that Debaryomyceshansenii (Candida famata) can colonize skin and mucous membranesof psoriatic patients and exacerbate psoriatic lesions ...
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Background: Psoriasis is an immune mediated skin disorderwhich is mainly characterized by abnormal proliferation anddifferentiation of keratinocytes. It is believed that Debaryomyceshansenii (Candida famata) can colonize skin and mucous membranesof psoriatic patients and exacerbate psoriatic lesions via toxins,antigens, and proteins. The aims of this study were to evaluateDebaryomyces hansenii colonization and its protein profile inpsoriatic patients.Method: Fifty-one patients with psoriasis vulgaris and 51 healthyindividuals were enrolled in the study. Skin and oral specimensfrom all participants were cultured on the CHROMagar Candidamedium. Isolated yeast like fungi were identified using thesequence of the D1/D2 domain of the 26S rRNA gene. Debaryomyceshansenii proteins were analyzed using Sodium Dodecyl Sulphate-Polyacrylamide gel electrophoresis.Result: Debaryomyces hansenii was only isolated from the oralcavity of 7.84% of the patients. The extracts obtained from variousDebaryomyces hansenii isolates had 45 protein bands, which rangedfrom 18 to >180 kDa. Secretory proteins were seen only in twoisolates, which ranged from 35 to100 kDa.Conclusion: Debaryomyces hansenii can colonize oral cavity ofpatients with psoriasis. In addition, various Debaryomyces hanseniiisolates have different somatic proteins, which may have a rolein provocation and exacerbation of psoriasis.