Jeelani Shazia; Lanker Audil; Jeelani Nasir; Masood Qazi; Fazili Tawheeda; Majid Huma
Volume 19, Issue 3 , 2016, , Pages 79-85
Abstract
Background: Onychomycosis (OM) is the infection of nails caused by a variety of fungi. As systemic antifungal treatment is necessary in the majority of patients, appropriate diagnostic techniques are important to ensure a correct diagnosis and treatment. Objective: This study was carried out to evaluate ...
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Background: Onychomycosis (OM) is the infection of nails caused by a variety of fungi. As systemic antifungal treatment is necessary in the majority of patients, appropriate diagnostic techniques are important to ensure a correct diagnosis and treatment. Objective: This study was carried out to evaluate the clinical, mycological, and histological aspects of onychomycosis in detail. Methods: A cross-sectional study was carried out on 216 patients with a high degree of clinical suspicion for OM. Clinical details including the site, morphological type, and associated diseases were noted in each case. The specimens obtained were subjected to three diagnostic tests: direct microscopy, fungal culture, and histopathological examination using PAS staining. Results: OM was more common in young adults in the age group 20-40 years (40.2%). In the majority (46.3%) of the cases, the duration of the disease was ≤ 1year. Distal and lateral subungual onychomycosis was the most common (73.6%) clinical type. A high incidence of toe nail onychomycosis (68.5%) was noted in our study. Dermatophytes were the most common etiological agent. Among the dermatophytes, Trichophyton mentagrophytes was the most common isolated fungus. Histopathological examination using PAS staining (HPE-PAS) showed the highest sensitivity of 91.6% among the three tests. Conclusion: Dermatophytes are the main agents responsible for OM in this region with T. mentagrophytes being the most common isolate. This study also demonstrated the importance of performing routine histopathology in addition to direct examination and culture for the diagnosis of onychomycosis.
Jowkar Farideh; Saki Nasrin; Derayesh Mohmmad; Aslani Fatemeh Sari
Volume 19, Issue 2 , 2016, , Pages 54-56
Abstract
Tinea incognito is a dermatophyte infection of the skin with an atypical presentation attributed to inappropriate treatment with immunosuppressive medications. In thi
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Tinea incognito is a dermatophyte infection of the skin with an atypical presentation attributed to inappropriate treatment with immunosuppressive medications. In thi
Mohammad Dehghan; Sepideh Hajian; Nazila Alborzi; Afsaneh Borgheyee; Amir Hussein Noohi
Volume 12, Issue 1 , 2009, , Pages 13-15
Abstract
Background: Dermatophytes are a group of closely related fungi that invade keratinized tissues (skin, hair and nails) of humans and animals and produce infections called dermatophytosis. Our objective was to determine causative agents of dermatophytosis in Gorgan, North of Iran. Methods: Data was based ...
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Background: Dermatophytes are a group of closely related fungi that invade keratinized tissues (skin, hair and nails) of humans and animals and produce infections called dermatophytosis. Our objective was to determine causative agents of dermatophytosis in Gorgan, North of Iran. Methods: Data was based on collecting specimens from 1108 patients clinically suspected to have fungal infection during five years from 2003 through 2007. Specimens were collected from hair, nail and skin and were investigated by direct examination and cultured in Sabouraud dextrose agar. Fungal colonies were identified by macroscopic and microscopic examination and supplementary tests. Results: 351 samples out of 1108 were positive for dermatophytes and 277 ones had positive cultures. Epidemophyton floccosum was the most frequent species (70.4%) followed by Trichophyton rubrum (14.5%) and Microsporum audouinii (7.2%). Regarding the location of the lesions, groin and nails were the most frequent sites that developed dermatophytosis in the majority of the patients. Conclusion: Dermatophytosis is probably still one of the most infectious diseases in Iran. The anthropophilic (E.fluoccosum) and zoophilic (T. rubrum) species were the most common causes of dermatophyte of tinea in Gorgan, north of Iran. The frequency of tinea was higher in females and tinea cruris showed a remarkably increasing rate and can be an important public health issue in Gorgan.
Mirhendi Seyed Hossein; Hedayati Mohammad Taghi; Omidi Khoshghadam; Zand Niloofar Jalali; Didehdar Mojtaba; Afshar Parvaneh
Volume 10, Issue 3 , 2007, , Pages 73-228
Abstract
Background and aim: Dermatophytosis (tinea, ringworm) is the infection of skin, hair or nail that is caused by various keratinophilic fungi (dermatophytes). Dermatophytosis is a common infection throughout the world including all parts of Iran. As conventional laboratory procedures for identification ...
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Background and aim: Dermatophytosis (tinea, ringworm) is the infection of skin, hair or nail that is caused by various keratinophilic fungi (dermatophytes). Dermatophytosis is a common infection throughout the world including all parts of Iran. As conventional laboratory procedures for identification of different dermatophytes are slow or lack specificity, more rapid and reliable methods are still required.Materials and methods: Dermatophyte fungi were isolated from patients with dermatophytosis and preliminarily identified by macroscopic and microscopic morphological criteria. Total cellular DNA was extracted from isolates using conical grinder. ITS1-5.8s-ITS2 region of rDNA region was amplified by the universal fungal primers ITS1 and ITS4 and digested with Eco RII enzyme.Results: 650-750 bp band was produced , as expected. Digestion of the PCR products with the restriction enzyme EcoRII produced different electrophoretic pattern and allowed us the identification and differentiation of common pathogenic dermatophytes including Trichophyton rubrum, T. interdigital, T. mentagrophytes, T. tonsurans, T.violaceum, T. schoenleinii, T. verrucosum, M.canis, M.gypseum and Epidermophyton floccosum. Conclusion: It seems that this PCR-restriction enzyme (PCR-RE) profile is a rapid and reliable tool for discrimination of important dermatophytes and can be an applicable method in reference medical mycology laboratories for diagnostic, as well as for large-scale epidemiological purposes.
