Background: Rosacea is a chronic skin disease with an unknown etiology. Some reports have suggested an increased prevalence of Helicobacter pylori infection in rosacea patients, but it is controversial. This study was designed to compare the prevalence of H. pylori and serological cag A+ species between the rosacea patients and the healthy control group. Methods: This case-control study was performed on 30 rosacea patients and 60 healthy individuals as the control group. The results were reported based on clinical and serological enzymelinked immunosorbent assay IgG antibody and cag A examination in two groups. Results: Mean age of the rosacea patients and the control group was 45.8 ± 14.05 and 41.4 ± 12.3 years respectively and 56.7% of the patients and 86.2% of controls were infected by H. Pylori (P=0.002). Furthermore, cag A+ was seen in 53.5% of the patients and 50% of the controls (P=0.23). Conclusion: This study showed a reduction in the prevalence of Helicobacter Pylori in acne rosacea patients compared to other studies in the world. The authors believe that it is necessary to conduct more studies to demonstrate the exact prevalence of this organism in Iranian rosacea patients.