Background: Vitiligo is a common disease. Its etiology is not known, but 30% of patients have a positive family history. Studies have been done on the influence of ABO and Rh blood groups on susceptibility to some diseases. Objective: The purpose of this case-control study was to evaluate the association of ABO and Rh blood groups with vitiligo. Patients and Methods: This study was performed on 200 vitiligo patients referred to skin clinics of Razi Hospital. The blood groups of patients were determined and compared with 200 control subjects and the general blood group distribution in Iran (Published by Iran Blood Transfer Organization). Results: There was no specific association established between vitiligo and ABO and Rh blood groups. Blood group B showed the most difference but still it was not significant (P>0.05). Conclusion: There was no association with the ABO and Rh blood group in vitiligo patients.