Background: Curcumin (diferuloylmethane) is one of the most
active components of turmeric. This herbal compound has antiinflammatory
and positive wound-healing impacts. The principal
objective of this study was to evaluate the impacts of curcumin
nanoliposomes on cell viability and motility of mouse fibroblast
NIH 3T3 cells and its wound healing effects on second-degree
skin burns in BALB/c mice.
Methods: Mature male BALB/c mice (n = 48) were divided
into 4 groups (n = 12 per group). Group one received curcumin
nanoliposome ointment; the positive and negative control groups
(groups 2&3) were treated with silver sulfadiazine and placebo,
respectively, and group four (sham) received no treatment. The
burn wound was created by a metal device with a diameter of 1
cm. Animals received treatment twice daily. On days 4, 7, 10, and
14, deep anesthesia and a biopsy of the wound were performed,
and a microscopic study and MTT assay were carried out.
Results: Cellular studies on mouse fibroblast NIH-3T3 cells
showed that low-dose curcumin nanoliposomes increased cell
proliferation and motility at 8, 12, and 24 hours in comparison
with the control group. In tissue samples of mice treated with
curcumin nanoliposome (day 14), less inflammation was observed,
while granulation tissue formation, fibroblast proliferation,
epithelialization, and collagen fiber synthesis increased significantly
compared with the control groups.
Conclusion: Our study indicates the positive effects of curcumin
nanoliposomes on the motility process of mouse fibroblast NIH-
3T3 cells (in vitro) and on the inflammatory and proliferative
phases (in vivo) of burn wound healing in mice.